FIGURE 6.

nhx1Δ phenocopy screen of single gene deletions. a, distribution of functional categories (upper) or cellular location (lower) as annotated by the MIPS Database of genes identified by mutant growth sensitivity to at least one (≥1) or more (≥2, ≥3) of four conditions as described under “Results.” *, p < 0.01. Note that functions and locations that are significantly enriched closely overlap with those of Nhx1, including cellular transport (function) and the endosome/vacuole (location). b, increasing selectivity of mutants with multiple shared nhx1Δ phenotypes. Of the ResGen yeast collection of 4828 viable single gene deletions, 1825 strains shared at least one of four phenotypes with nhx1Δ. Most had only one phenotype, with the number of mutants sharing multiple phenotypes falling steeply. Only two mutants (las17Δ and rcy1Δ) shared all four nhx1Δ phenotypes under the screen conditions.