FIGURE 5.

Direct binding of Tim44 to pHsp60. The interaction between Tim44 and pHsp60 was analyzed using cross-linking with DSS essentially as described in Fig. 1. A, the interaction of Tim44 (1.5 μm) with increasing concentrations of pHsp60. B, the interaction of 1.5 μm Tim44 with 1.5 μm pHsp60 in the presence of increasing NaCl concentrations. C, the interaction of 2 μm Tim44 with 0.9 μm pHsp60 in the presence of 1 mm ADP and increasing concentrations of mHsp70. In this experiment, the DSS concentration was 0.2 mm.