Table 2.
Dimensions and volumes of leaf lamella-cell lumina of Polytrichum formosum at different states of hydration
| State of hydration | Lumen shape | Lumen dimensions (μm) | Lumen volume (μm3) | Volume reduction (%) |
|---|---|---|---|---|
| Fully hydrated (control) | Approx. spherical | 8·5 diam. | 320 | – |
| Dehydrated | Concave disc | 8·0 diam., 6·0 edge depth,2·8 centre depth | 215 | 35 |
| 0·5 h rehydration | Concave disc | 8·0 diam., 6·0 edge depth,3·8 centre depth | 245 | 23 |
| 2·0 h rehydration | Approx. spherical with slightly concave outer faces | 8·5 µm diam. | 310 | 5 |
| 12 h, 24 h, 48 h rehydration | Approx. spherical | 8·5 µm diam. | 320 | – |
Data for the rehydrating samples are based on measurements from 30 cells under TEM, for fully hydrated samples a combination of 30 cells under the TEM and 50 cells observed directly by light microscopy, and for the dehydrated samples a combination of 30 cells under the TEM prepared by conventional and anhydrous fixation and 50 cells mounted in immersion oil.