Figure 4.

C/EBPβ stimulates the transcription of autophagy genes through direct promoter occupancy. (A) Transcriptional assays using indicated promoter luciferase constructs in the presence of vector (open), 25 ng (grey), or 100 ng (filled) of C/EBPβ expression plasmid. Predicted C/EBPβ binding sites are indicated with solid bars. Data represent mean±s.e. Student's t-test was applied. ^*P<0.01 (vector versus 25 ng C/EBPβ); ^**P<0.001 (vector versus 100 ng C/EBPβ). (B) ChIP assay using control IgG (open) or C/EBPβ (filled) antibodies. Relative enrichment was determined by qPCR.