Figure 9.

C/EBPβ is essential for circadian autophagy regulation in the liver. (A) qPCR analysis of hepatic genes in mice transduced with control (Scrb, filled diamond) or siC/EBPβ (siC, open square) adenoviruses harvested at indicated time points. Data represent mean±s.d. (B) Immunoblots of liver lysates in mice from (A) at indicated time points. (C) In vivo autophagy flux assay was performed in mice transduced with control (Scrb) or siC/EBPβ (siC) adenoviruses at ZT6-9. Pooled samples from 3 to 5 mice per group were analysed. (D) Model depicting circadian autophagy regulation through C/EBPβ. Note that C/EBPβ receives both circadian and nutritional input and coordinately regulates the program of autophagy gene expression.