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The A36 WD/E motifs are important for the cell-to-cell spread of the virus. (A) Representative fluorescence images of plaques produced by the WT, WE/AA, WD/AA and WDWE/AAAA viruses in the A36–YdF–YFP background at 48 h post-infection. Scale bar=100 μm. (B) Quantification of plaque sizes produced by the indicated viruses at 48 h post-infection. A P-value of <0.001 is indicated with *** and error bars represent s.e.m. from 30 plaques.
