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. 2011 Sep 13;30(22):4523–4538. doi: 10.1038/emboj.2011.326

Figure 4.

Figure 4

Bipartite tryptophan motifs of Calsyntenin can functionally replace A36. (A) Schematic representation of hybrid protein in which the region following the A36 TM domain (residue 32 onwards) is replaced with residues 879–971 of Calsyntenin. (B) Images showing the spread of the indicated recombinant A36–TM–CSTN1–GFP mutant viruses detected with anti-A27 (virus) from their perinuclear site of assembly to the cell periphery at 11 h post-infection. Scale bar=10 μm. (C) The graph shows quantification of extracellular B5 labelling for the indicated viruses. A P-value of <0.001 is indicated with *** and error bars represent s.e.m. from 50 cells in three independent experiments. (D) Images showing recruitment of RFP-tagged KLC1 and KLC2 to the WT A36–TM–CSTN1–GFP virus (GFP) co-labelled with DAPI to identify the viral genome. Scale bars=2 μm. (E) Representative fluorescence images of plaques produced by the A36–YdF–YFP, A36–TM–GFP and A36–TM–CSTN1–GFP viruses at 48 h post-infection. Scale bar=100 μm. (F) Quantification of plaque sizes produced by the indicated viruses at 48 h post-infection. A P-value of <0.001 is indicated with *** and error bars represent s.e.m. from 30 plaques.