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. 2011 Sep 13;30(22):4523–4538. doi: 10.1038/emboj.2011.326

Figure 6.

Figure 6

Multiple bipartite tryptophan motifs can rescue vaccinia transport. (A) Images showing the spread of the indicated recombinant A36–TM–SKIP–GFP viruses detected with anti-A27 (virus) from their perinuclear site of assembly to the cell periphery at 11 h post-infection. Scale bar=10 μm. (B) Quantification of viral spread to the cell periphery for the indicated recombinant A36–TM–SKIP–GFP viruses. A P-value of <0.001 is indicated with *** and error bars represent s.e.m. from 50 cells in three independent experiments. (C) Representative immunofluorescence showing the spread of A36–TM–ATF6–GFP and A36–TM–BSDC1–GFP viruses detected with anti-A27 (virus) from their perinuclear site of assembly to the cell periphery at 11 h post-infection. Scale bar=10 μm. (D) Quantification of viral spread to the cell periphery as detected by staining for extracellular B5 in the absence of permeabilization for the indicated recombinant viruses. A P-value of <0.001 is indicated with *** and error bars represent s.e.m. from 50 cells in three independent experiments. (E) Quantification of plaque sizes produced by the indicated recombinant viruses at 48 h post-infection. A P-value of <0.001 is indicated with *** and error bars represent s.e.m. from 30 plaques. (F) Quantification of transient rescue of viral spread to the cell periphery at 10 h post-infection. A P-value of <0.001 relative to the A36–TM control is indicated with *** and error bars represent s.e.m. from 50 cells in three independent experiments.