Figure 7.

(A) Images showing recruitment of RFP-tagged KLC1 and KLC2 to the different recombinant virus particles. Purple arrows indicate virus particles (IEV) that show good recruitment of the indicated KLC isoform. The white arrows highlight weak recruitment of KLC2 by the A36–TM–BSDC1–GFP virus and yellow arrows indicate A36–TM–ATF6–GFP virus that cannot recruit KLC1. Scale bars=2 μm. (B) Western blot analysis of co-immunoprecipitation with the indicated antibodies reveals that FLAG–ATF6 associates with HA–KLC2 but not HA–KLC1 (left panel). In contrast, GFP–PARC interacts with HA–KLC1 but not HA–KLC2 (right panel). (C) Western blot analysis of co-immunoprecipitation experiments between GFP-tagged SKIP, BSDC1, FAM63B, LDLRAP1, PRKAG3, RASSF8 and RIC3 with either HA–KLC1 (left panel) or HA–KLC2 (right panel) reveals the candidate proteins can bind both isoforms or have distinct binding preferences for KLC1 or KLC2.