Skip to main content
. 2011 Sep 13;30(22):4628–4641. doi: 10.1038/emboj.2011.331

Figure 6.

Figure 6

miR-101 directly targets STMN1, RAB5A and ATG4D. (A) Predicted binding between miR-101 and the 8mer seed matches in STMN1, RAB5A and ATG4D 3′UTRs. (B, C) miR-101 regulates STMN1, RAB5A and ATG4D 3′UTR reporters. Luciferase reporter assays 24 h after transfection with indicated pGL3 firefly plasmids and a renilla transfection control plasmid, co-transfected with miR-101, LNA miR-101 or relevant scramble controls. Data shown are the mean±s.d. of four replicates and are representative of two or three independent experiments. *P<0.05, **P<0.005, ***P<0.0005. (D) miR-101 decreases Stathmin and RAB5A protein levels. Western blot analysis 48 or 72 h after transfection with miR-101 or scramble control. The Stathmin and RAB5 bands were quantified relative to the vinculin loading control using ImageJ software and the relative quantifications are shown. The data are representative of three independent experiments.