Figure 2.

Jarid1b is not required for differentiation of neural stem cells derived from brain. (A) Schematic representation of experimental outline. DIV: days in vitro. (B) Immunoblots of conditional Jarid1b neural stem cells (NSCs) 72 h after induction of Cre-mediated deletion by 4OHT. (C) Phase-contrast images of Jarid1b^F/F (−4OHT) and Jarid1b^−/− (+4OHT) NSCs at 0 and 8 days of differentiation. (D) Expression of neuronal marker genes during differentiation of Jarid1b^F/F (−4OHT) and Jarid1b^−/− (+4OHT) NSCs determined by quantitative RT–PCR analysis (normalized to β-actin levels). (E) Immunofluorescence analysis of the neural stem cell marker Nestin performed on an automated screening platform.