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. 2011 Nov 1;30(23):4777–4789. doi: 10.1038/emboj.2011.393

Figure 6.

Figure 6

Smad3 is ubiquitinated at the C-terminal (MH2) domain. (A) Schematic representation of Smad3 structure. Four potential Ub acceptor lysines are denoted as A, B, C, or D. (B) IP-western blot analyses of various Smad3 fragments in Smurf2−/− MEFs. FL, full-length Smad3; N, MH1 domain; NL, MH1 domain+linker region; LC, linker region+MH2 domain; C, MH2 domain. (C) IP-western blot analyses of the C-terminal Ub acceptor site lysine mutants in Smurf2−/− MEFs. The arrow indicates the dual mono-ubiquitinated Smad3. Figure source data can be found with the Supplementary Information.