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. 2011 Nov 15;30(24):4860–4873. doi: 10.1038/emboj.2011.401

Figure 7.

Figure 7

UCP2 regulates ROS, hPSC viability, and differentiation. (A) Representative ROS determination in transduced HSF1 cells by 2‐dihydroethidium staining and flow cytometry (n=3). (B) Apoptosis staining and flow cytometry. Data are plotted in the right panel. Data are expressed as mean±s.d. (n=3), * P<0.05. (C) ROS determination in transduced HSF1 cells with or without differentiation induction by media replacement for 2 days (SPD2). Panel (iii) is an overlay of (i, ii); panel (vi) is an overlay of (iv, v); panel (vii) is an overlay of (i, iv); and panel (viii) is an overlay of (ii, v). (D) EB formation assay with transduced HSF1 cells. Data are expressed as mean±s.d. (n=3), * P<0.05. (E) mRNA expression of developmental genes in transduced HSF1 cells. Data are expressed as mean±s.d. (n=3), * P<0.05.