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. 2011 Sep 16;30(24):4955–4969. doi: 10.1038/emboj.2011.339

Figure 3.

Figure 3

Selective activity-dependent requirement for syndapin I during synaptic vesicle recycling in hippocampal cultures. (A) Hippocampal cultures were loaded and unloaded with FM1-43 dye using the protocol displayed. (B, C) Time course of the average response of WT (B) and syndapin I KO (C) nerve terminals loaded with FM1-43 is displayed. KCl evoked unloading is shown by bar. The amount of dye unloaded after Stim1 (300 stimuli) and Stim2 (800 stimuli) yields ΔF1 and ΔF2. (D, E) Quantification of mean dye release after 300 stimuli (ΔF1, D) and after 800 stimuli (ΔF2, E) revealed a trend to reduced dye uptake after 300 stimuli (D) and significantly decreased dye uptake after 800 stimuli (E) in syndapin I KO hippocampal neurons, suggesting defective activity-dependent SV endocytosis in the absence of syndapin I. (F, G) Reduced SV turnover in individual nerve terminals shown by cumulative histogram of the ΔF2/ΔF1 ratios of individual synapses (F) and the averaged ΔF2/ΔF1 response (G) suggesting defective activity-dependent SV endocytosis in the absence of syndapin I. *P<0.05; t-test.