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. 2011 Sep 16;30(24):4955–4969. doi: 10.1038/emboj.2011.339

Figure 5.

Figure 5

Strongly increased dynamin extractability in syndapin I KO mice. (A) Equal amounts of Triton X-100-soluble fractions of either genotype subjected to quantitative western blot analyses normalized to the corresponding actin signal on the same membrane. Deviations of the mean in KO samples (set to 0) to the mean in WT samples (red column) are expressed in percent. Data represent mean±s.e.m. For dynamins, a highly statistically significant increase in Triton X-100 extractability was observed (dynamin I: 39±7%, dynamin II: 50±7%, dynamin III: 105±16%). A similar increase was observed upon normalization to tubulin. Mann–Whitney U-test; **P<0.01, ***P<0.001. (B) Primary hippocampal neurons (21 DIV) were subjected to Triton X-100 extraction and subsequent immunocytochemistry. Dynamin III levels were reduced to background signal in syndapin I KO cultures, whereas a reasonable signal was still present in WT cultures. Anti-ProSAP2 immunostaining served as control, this synaptic scaffold protein was not extracted. Scale bar, 20 μm.