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. 2011 Dec 20;6(12):e29050. doi: 10.1371/journal.pone.0029050

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A. Representative real-time, generic MLV protease (pro) amplification plot. Blue lines, XMRV RNA standard extracted from 22Rv1 cell culture supernatants from 10⁶–10⁰ copies per reaction; burgundy lines with triangles, 4/16 (25%) water only controls tested positive for MLV pro sequences using the ABI TaqMan Fast 1-step Master Mix; bright green line, RFU, relative fluorescent units. B. Representative gel image showing nested PCR detection of 208-bp MLV polymerase (pol) sequences in water only control reactions using Finnzymes RobustI AMV RT. Lanes 1–16, water only controls; lanes 17–20, XMRV RNA extracted from 22Rv1 cell culture supernatants from 10³, 10², and 10 copies per reaction, respectively; M. molecular weight marker.