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. 2011 Dec 20;6(12):e29050. doi: 10.1371/journal.pone.0029050

Table 2. Contamination of commercial human DNAs with mouse DNA.1 .

Manufacturer DNA Specimen Lot no. pro (copies/rxn) gag pol mtDNA 2 (copies/µg) IAP 2 (copies/µg)
Biochain Human leukocyte DNA A807119 8,000 Pos Pos 232,000 39,200
Human leukocyte DNA3 B210149 Neg Neg Neg 1–10 1–10
Dementia DNA B409046 10 Pos Pos 46 120
Parkinson's DNA B303007 1–10 Pos Pos 1430 877
Alzheimer's DNA B111073 10–20 Pos Pos 6 1120
Multiple sclerosis DNA B409045 1–10 Pos Pos 52 16
Raji DNA NA4 Neg Pos Pos 58 10–20
Jurkat DNA NA Neg Pos Pos 2 10–20
K562 DNA NA Neg ND Neg 6 Neg
MCF-7 DNA NA Neg ND Neg Neg 10–20
Sigma-Aldrich Male placental DNA 88F3847 Neg Neg Neg Neg Neg
Female placental DNA NA Neg Neg Neg Neg Neg
1

1 ug of the human DNAs were tested using quantitative (qPCR) pro and nested pol and gag PCR assays. Copies/µg DNA is provided in parentheses for specimens with evidence of contamination.

2

Mouse DNA contamination was detected using mitochondrial DNA (mtDNA) and intracisternal A particle (IAP) PCR qPCR assays.

3

Second lot of human DNA sample received from Biochain after informing them of the contamination results.

4

NA, information not available.