Table 2. Contamination of commercial human DNAs with mouse DNA.1 .
Manufacturer | DNA Specimen | Lot no. | pro (copies/rxn) | gag | pol | mtDNA 2 (copies/µg) | IAP 2 (copies/µg) |
Biochain | Human leukocyte DNA | A807119 | 8,000 | Pos | Pos | 232,000 | 39,200 |
Human leukocyte DNA3 | B210149 | Neg | Neg | Neg | 1–10 | 1–10 | |
Dementia DNA | B409046 | 10 | Pos | Pos | 46 | 120 | |
Parkinson's DNA | B303007 | 1–10 | Pos | Pos | 1430 | 877 | |
Alzheimer's DNA | B111073 | 10–20 | Pos | Pos | 6 | 1120 | |
Multiple sclerosis DNA | B409045 | 1–10 | Pos | Pos | 52 | 16 | |
Raji DNA | NA4 | Neg | Pos | Pos | 58 | 10–20 | |
Jurkat DNA | NA | Neg | Pos | Pos | 2 | 10–20 | |
K562 DNA | NA | Neg | ND | Neg | 6 | Neg | |
MCF-7 DNA | NA | Neg | ND | Neg | Neg | 10–20 | |
Sigma-Aldrich | Male placental DNA | 88F3847 | Neg | Neg | Neg | Neg | Neg |
Female placental DNA | NA | Neg | Neg | Neg | Neg | Neg |
1 ug of the human DNAs were tested using quantitative (qPCR) pro and nested pol and gag PCR assays. Copies/µg DNA is provided in parentheses for specimens with evidence of contamination.
Mouse DNA contamination was detected using mitochondrial DNA (mtDNA) and intracisternal A particle (IAP) PCR qPCR assays.
Second lot of human DNA sample received from Biochain after informing them of the contamination results.
NA, information not available.