Figure 4. Flow cytometric analysis of rGP63-specific IFN-γ producing CD4⁺ and CD8⁺ T cells.

BALB/c mice were primed with liposomal rGP63 plus MPL-TDM, followed by boosting with either rGP63 alone, in association with MPL-TDM, or liposomes, or both before challenge infection. Splenocytes were isolated from vaccinated mice and the expression of IFN-γ producing CD4⁺ (A) and CD8⁺ T (B) was studied in the presence of rGP63 (5 µg/ml). Data represent the mean of triplicate wells ± SE of three individual mice per group. **p<0.01, ***p<0.001 assessed by two-tail t test.