Figure 7. Ovalbumin taken up by mouse PMNs can be transferred to DC that in turn cross-present antigen.

(A) Schematic representation of the experimental conditions in which OVA was added to bone-marrow-derived PMNs from BALB/c mice which were extensively washed 2 h later and subsequently subjected or not to UV-irradiation. Then PMNs were co-cultured overnight with DC derived from the bone marrow of C57BL/6 mice. The next morning CFSE-loaded OT-1 or OT-2 TCR-transgenic T cells were added to the cultures. (B) FACS histograms showing CFSE dilution at 72 h of culture in the indicated conditions. DC pulsed with the cognate OVA peptides recognized by OT-1 and OT-2 T lymphocytes were used as positive controls. The experiment shown is representative of at least three similarly performed.