Table 1. Description of the 20 microsatellite loci used in this study for Leishmania donovani complex.

Locus	Locus abbreviation	GenBank Accession no.	Allele size (bp)	Chromosome	Ta (°C)	Na	Hs	F IS
DPB1	D1	AF182167	143–147	8	59	4	0.544	0.970
DPB2	D2	AF182167	235–245	8	59	6	0.526	0.688
HG	HG	AF170105	187–203	12	55,2	6	0.725	0.887
Rossi1	R1	X76394	101–115	8	59	5	0.534	0.724
Rossi2	R2	X76393	143–163	14	57	5	0.657	0.077
LIST7021*	L21	AF427869	216–228	36	54	3	0.423	0.884
LIST7024*	L24	AF427872	198–222	30	59	7	0.786	0.020
LIST7025*	L25	AF427873	168–212	10	56	8	0.373	0.122
LIST7026*	L26	AF427874	207–221	13	56	2	0.300	0.672
LIST7027*	L27	AF427875	185–191	26	59	4	0.501	0.967
LIST7028*	L28	AF427876	151–153	36	58	2	0.450	0.016

The following parameters are described: name, abbreviation, Genebank accession number, allele size (bp), chromosome localization, thermocycling conditions (annealing temperature, T_a), genetic variation (alleles number), Na; average estimate within-sample gene diversity H _S, and deviation from panmixia measured as F _IS. The loci noted by ‘*’were developed by Jamjoom et al. [26].