FIG. 1.

Perfluoroalkyls increase liver tumor incidence, multiplicity and size in AFB₁- and MNNG-initiated trout. (A–C) Liver tumor incidence and multiplicity (males and females). (D–F) Average liver tumor size ± SE. Trout were initiated with 10 ppm AFB₁ at 10 (A, D) or 15 weeks of age (B, E) or with 35 ppm MNNG at 10 weeks (C, F). Details on experimental diets are provided in “Materials and Methods.” **p < 0.01 and ****p < 0.0001, significant difference in tumor incidence compared with CON diet (within each initiation group) as determined by logistic regression analysis (complete results in Supplementary tables 2 and 3). #p < 0.05 and ###p < 0.001, significant difference in tumor multiplicity or size compared with CON diet (within each initiation group) as determined by the Kruskal-Wallis test with Dunnett’s post hoc test for multiple comparisons. A color version of this figure is available in the online version of the article.