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. Author manuscript; available in PMC: 2012 Nov 3.
Published in final edited form as: Oncogene. 2011 Sep 19;31(18):2270–2282. doi: 10.1038/onc.2011.405

Figure 7. Activation of Wnt signaling decreases Sox2 expression in osteoblasts and osteosarcoma.

Figure 7

(A) Sox2 Expression in Wnt-treated cells. Western blot of Sox2 in osteosarcoma cells (mOS-482), immortalized osteoblasts (OB1). and primary osteoblasts transduced with empty vector (-) or Sox2 (+) lentivirus, after treatment with control or Wnt 3A conditioned medium for 48 hours. γ- tubulin was used as a loading control.

(B) Model for Sox2-Wnt antagonism in osteosarcoma. Undifferentiated osteosarcoma cells with high Sox2 expression and low Wnt pathway activity are in a stem-like, multipotent, self-renewing state.suggesting that Sox2 and Wnt pathway are mutually antagonistic in osteosarcoma development. Sox2 depletion or activation of Wnt signaling leads to a more differentiated, osteoblast-like state.