Table 1. Limiting dilution analysis of osteosphere-forming frequency of Sca-1HI Sox2HI and Sca-1LO Sox2LO cells from mOS-482.
Number of cells/well | Number of wells plated | Number of wells with osteospheres | |
---|---|---|---|
Sca-1HI Sox2HI | Sca-1LO Sox2LO | ||
1000 | 24 | 24 | 24 |
100 | 84 | 84 | 78 |
10 | 84 | 34 | 5 |
1 | 84 | 4 | 0 |
Sphere-forming frequency (95% CI) | 1/18 (1/14-1/24) | 1/50 (1/40-1/64) | |
P value | <0.0001 |