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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Cancer Res. 2011 Oct 12;9(12):1718–1731. doi: 10.1158/1541-7786.MCR-10-0210

FIGURE 4.

FIGURE 4

Effects of TNFR2 over-expression or siRNA-mediated knockdown on colon cancer cell proliferation. (A) Histogram of [3H]-thymidine incorporation into DNA as a measure of COLO205 or SW480 cell proliferation after 24-hour over-expression of TNFR2. TNFR2 over-expression significantly increased cell proliferation (*p ≤ 0.05 compared to empty vector). (B) SW480 cells were treated with control, TNFR1, or TNFR2 siRNA for 24 hours. Histogram shows fold change in expression of TNFR1 or TNFR2 mRNA. TNFR2 siRNA led to a 60% decrease in TNFR2 mRNA, with no effect on TNFR1 mRNA (*p ≤ 0.05 compared to other treatments). (C) Untreated SW480 cells transfected with TNFR2 siRNA exhibit a modest, but significant (*p ≤ 0.05) decrease in [3H]-thymidine incorporation into DNA. (D) Cytokine-treated SW480 cells with TNFR2-knockdown exhibited a significant decreases cell number measured by WST-1 assay, while TNFR1 knockdown significantly increased cell number (*p ≤ 0.05 compared to control siRNA, **p≤ 0.05 compared to cells treated with TNFR1 siRNA). (n = 3).