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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Neurochem. 2011 Nov 24;120(2):334–346. doi: 10.1111/j.1471-4159.2011.07566.x

Fig 2. NAD+ and NAM protect neurons after excitotoxic glutamate stimulations.

Fig 2

(A–B)Effect of NAD+ and NAM on cell viability after 50 μM and 100 μM glutamate stimulations. Neurons were treated with glutamate for 3 h in the absence and in the presence of various concentrations of NAD+ (A) and NAM (B). NAD+ and NAM were continuously present after glutamate stimulation for a total time of 24 h. Neuronal viability was then determined by MTT assay. (C) Summary of PI staining confirming the neuronal protective effect of NAD+ and NAM after glutamate stimulations. The percentage of PI+ cells was calculated based on the total number cells determined by Dapi-stained nuclei and was normalized to the values in the absence of NAD+ and NAM. Data from (A–C) were the mean values from 5–6 independent experiments; for each experiment, 3–4 parallel assays were performed and averaged as a single value for that experiment. *t-test with p<0.05; **t-test with p<0.005 vs no NAD+ or NAM treatment for (A–C).