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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: J Neurochem. 2011 Nov 11;120(2):239–247. doi: 10.1111/j.1471-4159.2011.07539.x

Figure 1. CNTF suppresses TH via gp130.

Figure 1

A. A representative western blot showing TH content in wild type (WT) and gp130−/− sympathetic neurons following 5 days in 100ng/ml CNTF. The blot was stripped and re-blotted for GAPDH to control for protein loading. B. Quantification of TH content assayed in triplicate by immunoblot. TH was decreased in WT but not gp130−/− neurons. Data are mean ± SEM, averaged from 3 independent experiments; ***p<0.001. C. Phospho-ERK1/2 (p-ERK1/2) and phospho-STAT3 (p-STAT3) were identified by immunoblot in M17 neuroblastoma cells, WT mouse neurons and gp130−/− neurons treated with CNTF for 15 minutes. Membranes were stripped and re-probed for total ERK as a control. Data shown are representative of three experiments assayed in triplicate.