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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: Clin Cancer Res. 2011 Oct 28;17(24):7693–7703. doi: 10.1158/1078-0432.CCR-11-1488

Figure 1. Characterization of bone sarcoma xenografts.

Figure 1

A. Representative images of HE-staining, CD34, Ki-67 and HIF-1αexpression in OS-1, OS-33, EW-5 and EW-8 xenografts. HIF-1α staining is cytoplasmic in normoxia, and nuclear in response to hypoxia. Inset demonstrates ×400 magnification of boxed area to visualize HIF-1α expression in more detail (note nuclear staining in EW-5 and EW-8 tumors compared to predominantly cytoplasmic staining in OS-1 and OS-33 tumors). Three different xenografts from each tumor type were used for each staining; representative images are shown. All images are ×50 magnification unless stated otherwise, haematoxylin counterstain.

B. Representative images demonstrating IGF-1R expression levels in OS-1, OS-33, EW-5 and EW-8 xenografts. Localization of necrotic and hypoxic areas was derived from Ki-67 and HIF-1α staining patterns (Figure 1A). Three different xenografts from each tumor type were submitted to IHC, representative images are shown. All images are ×400 magnification, haematoxylin counterstain.

Arrows indicate membranous IGF-1R expression in IGF-1R positive xenografts.