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. Author manuscript; available in PMC: 2013 Jan 30.
Published in final edited form as: Immunol Lett. 2011 Oct 20;141(2):220–226. doi: 10.1016/j.imlet.2011.10.008

Figure 2.

Figure 2

4-1BB and 4-1BBL could be detected on VACV-specific CD8 T cells and DC respectively following infection. a-b) WT mice were infected i.p with VACV-WR (2 × 105 PFU/mouse). a) On indicated days post-infection splenocytes were harvested and stained for CD8, CD44, B8R-tetramer, and 4-1BB. Top panel, Percentage of CD44-high expressing B8R-specific CD8 T cells. Bottom panel, Percentage of 4-1BB+ cells gating on CD8+ CD44-high B8R-tetramer positive cells. Quadrant settings based on isotype controls. b) On indicated days post-infection splenocytes were harvested and stained for CD8, CD11c, CD11b, and 4-1BBL. Representative histogram of 4-1BBL expression gated on CD8+CD11c+ cells.