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. Author manuscript; available in PMC: 2013 Jan 30.
Published in final edited form as: Immunol Lett. 2011 Oct 20;141(2):220–226. doi: 10.1016/j.imlet.2011.10.008

Figure 3.

Figure 3

VACV-specific memory CD8 T cell generation is independent of 4-1BB and 4-1BBL. WT, 4-1BB-/-, and 4-1BBL-/- mice were infected i.p. with VACV-WR (a: 2 × 103 PFU/mouse, or b-d: 2 × 105 PFU/mouse). 120 days after infection, splenocytes were stimulated with B8R peptide (a, b, c) or VACV subdominant peptides, A3L, A8R, A23R and B2R (d). Total numbers of CD8+CD44+B8R-tetramer positive cells (a, b) and CD8+IFN- + cells (c) were enumerated. (c) Representative plots of IFN- (left), TNF (middle), and IFN- /TNF (right), gating on CD8+CD62L- cells. Percentages positive are indicated. Data represent mean value ± sem from n=4 to 6 mice. Similar results were reproduced in two separate experiments.