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. Author manuscript; available in PMC: 2013 Jan 30.
Published in final edited form as: Immunol Lett. 2011 Oct 20;141(2):220–226. doi: 10.1016/j.imlet.2011.10.008

Figure 5.

Figure 5

Blocking 4-1BB and 4-1BBL interaction does not impair generation of VACV-specific memory CD8 T cells. (a-b) WT B6 mice were infected i.p. with VACV-WR (2 × 105 PFU/mouse) and treated with IgG or 4-1BBL neutralizing antibody (19H3) on days 0, 2, and 5 post infection. (c) WT B6 mice were infected i.p. with VACV-WR (2 × 105 PFU/mouse) and treated with IgG or 4-1BBL neutralizing antibody (19H3) on days 0, 2 and 5 (early block) or 7, 10 and 13 (late block). 35 days (a, b) or 120 days (c) after infection, splenocytes were stimulated with B8R peptide (a, c) or VACV subdominant peptides, A3L, A8R, A23R and B2R (b) to measure CD8+IFN- + cells (a-c), or CD8+ T cells were stained with B8R-tetramer (a, c). Data represent mean total numbers ± sem from n=4 to 6 mice. Similar results were reproduced in two separate experiments.