The percentage of eclosed flies from wild type (w1118) and miR-8 mutants (miR-8jk22/miR-8jk22) was determined after rearing flies at 18°C, 25°C or 29°C. Percent eclosion was determined by dividing the number of empty pupal cases by the total number of cases. The experiment was performed with 6 batches of flies per genotype/temperature combination and the mean percent eclosion was calculated. Error bars indicate SEM. Results are representative of three separate trials. Statistical analysis revealed a strong interaction between miR-8 and temperature (ANOVA, F2,30=138.75, p<0.001). Shared letters indicate no significant difference (Tukey post-hoc, α=0.05).