Figure 1.

RGMb deletion does not alter development of DRG neurons as revealed by in situ hybridization. A, RGMb is widely expressed in small and large DRG neurons of adult C57BL/6 mice as demonstrated by coexpression with the sensory neuron markers peripherin, TrkA and NF-200, visualized by fluorescent immunohistochemistry (right) and in situ hybridization (left). Arrows indicate cells that express peripherin, TrkA, or NF-200 (white arrows) and RGMb (yellow arrows). Scale bar, 100 μm. B, In situ hybridization revealed no difference between RGMb KO and WT LM in the expression of a variety of key markers of sensory neurons in P0 DRG.