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. 2011 Dec 14;31(50):18391–18400. doi: 10.1523/JNEUROSCI.4550-11.2011

Figure 3.

Figure 3.

Neurite outgrowth in axotomized RGMb KO DRG neurons is reduced and can be rescued by BMP-2. A, Fluorescent microscopy (anti-β-tubulin immunostaining) of dissociated DRG neurons from RGMb KO and WT LM mice. Fewer cultured DRG neurons from RGMb−/− mice form neurites, and neurites that do form are shorter than those from neurons derived from WT LMs (*p < 0.001). Addition of BMP-2 (50 ng/ml) to the RGMb−/− neurons reverses this effect (no significant difference compared with RGMb+/+). Scale bar, 250 μm. B, Fluorescent micrographs demonstrating that cultured whole DRG explants from RGMb−/− mice generate significantly fewer neurites than explants from WT LM mice and that this loss of function is reversed by the addition of BMP-2 (50 ng/ml). *p < 0.001, statistically significant difference from all other groups. Values represent mean ± SEM as shown in all graphs. Scale bar, 250 μm.