Figure 7.

Effect of BFA on the accumulation of green auto-fluorescent compounds. (A) Accumulation of green fluorescence compounds in ERE::P1 cells after treatment with BFA, induced (squares) or not induced (triangles) with estradiol. The y-axis indicates the percentage of cells with cytoplasmic (green line) GFBs or cell wall (red line) green fluorescence. (B) Epifluorescence and bright field microscopic image of ERE::P BMS cell 6 days after treatment with estradiol and 10 μg/ml of BFA indicating the tubular structures sometimes filled with green fluorescence that appear after the treatment with BFA.