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. 2011 Dec 19;208(13):2733–2746. doi: 10.1084/jem.20111155

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© 2011 Wesemann et al.

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Figure 7. — Assessment of Bcl6 and ID2 transcript levels in IBCs compared with splenic B cells. Quantitative PCR for Bcl6 (A) and ID2 (B) expression was performed on samples from unstimulated and αCD40+IL-4–activated splenic B cells and IBCs. (C) Quantitative PCR of Bcl6 expression in WT IBCs versus IBCs from mice heterozygous for the Iμ-Bcl6 transgene. Values were normalized to GAPDH. Shown are mean values ± SD of three independent experiments. The p-values were calculated using the two-tailed Student’s t test. (D) IBCs from WT mice (top row), and IBCs from Iμ-Bcl6 transgenic mice (bottom row) were stimulated with αCD40+IL-4. Cells were analyzed for IgG1 and IgE switching using the intracytoplasmic staining method outlined in Fig. 3 A. The experiment in D was repeated a total of three times from three independent samples per group and the mean IgE (E) and IgG1 (F) expression ± SD for are shown.