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. 2011 Dec 19;208(13):2641–2655. doi: 10.1084/jem.20110840

Figure 3.

Figure 3.

BMP7 up-regulates NDRG1 by activating p38 and p21. (A) Effect of 200 ng/ml BMP7 on senescence in PC3 mm was examined by SA–β-gal staining (n = 4). −, control vehicle. The inset shows representative images of three independent experiments. Bar, 100 µm. (B and C) Effect of 200 ng/ml BMP7 on p21 was measured by qRT-PCR and p21 reporter assay in PC3 mm (B and C) and DU145, C4, C4-2, and C4-2B cells (B; n = 3). Control, control vehicle. (D) PC3 mm cells were transiently transfected with dominant-negative Smad4 (DN-Smad4) or empty vector and then cultured with or without BMP7 for 48 h. Expression of p21 was assessed by qRT-PCR (n = 3). (E) PC3 mm cells were treated with or without 200 ng/ml BMP7 and/or 10 µM SB203580 (SB), and the expression of p21 was examined by qRT-PCR (n = 3). (F) PC3 mm cells that had either scrambled shRNA (scramble) or shRNA for p21 (shp21) were treated with or without BMP7, and the expression of p-p38, p38, p21, NDRG1, and β-tubulin was examined by Western blot. *, P < 0.05; **, P < 0.01; ***, P < 0.001. All experiments were performed three times independently, and representative data are shown. Results are shown as mean ± SEM.