Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 19;208(13):2641–2655. doi: 10.1084/jem.20110840

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Kobayashi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — BMP7 induces reversible senescence in CSCs through activation of p38, p21, and NDRG1. (A) CSCs isolated from PC3 mm were injected subcutaneously into nude mice, and the growth of tumors was monitored by BLI. (B) The CSCs were treated with the HS5-CM or BMP7, and the expression of p-p38, NDRG1, and β-tubulin was examined by Western blot. (C and D) The CSCs were treated with or without BMP7 and/or SB203580 (SB), and the expression of NDRG1 (C) and p21 (D) was examined by qRT-PCR (n = 3). *, P < 0.05; ***, P < 0.001. (E) Effect of BMP7 on the sphere forming ability of CSCs was measured. −/−/−, no treatment throughout; −/+/−, no treatment for 5 d, treated with BMP7 for 4 d, and no treatment thereafter; +/+/−, treatment for 9 d and no treatment thereafter (n = 5). *, P < 0.001 versus −/−/−; #, P < 0.001 versus −/−/−. (F) CSCs were cultured in the presence (+/+), absence (−/−), or withdrawal after treatment (+/−) of BMP7, and SA–β-gal staining was performed (n = 6). ***, P < 0.001. Bar, 10 µm. (G and H) The CSCs were treated with BMP7, and the expression of NDRG1 (G) and p21 (H) was measured by qRT-PCR. −/−, no treatment control; +/+, continuous treatment with BMP7 for 96 h; +/−, 48-h treatment followed by 48-h withdrawal of BMP7 (n = 3). *, P < 0.05; **, P < 0.01. (I) A similar experiment was performed as in E for CSCs from PC3 mm/Tet-NDRG1 with (+) or without (−) induction of NDRG1, followed by assaying sphere formation. −/−/−, no induction throughout; −/+/−, no induction for 5 d, induction for 3 d, and no induction thereafter; +/+/−, induction for 8 d and no treatment thereafter (n = 5). *, P < 0.001 versus −/−/−; #, P < 0.001 versus −/−/−. (J) A similar experiment was performed as in F for CSCs from PC3 mm/Tet-NDRG1. They were cultured with (+/+) or without (−/−) induction or withdrawal after induction of NDRG1 (+/−), and SA–β-gal staining was performed (n = 8). ***, P < 0.001. The expression of NDRG1 and β-tubulin was examined by Western Blot (top). The experiment in A was performed twice, experiments in B–J were performed three times independently, and representative data are shown. Results are shown as mean ± SEM.