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. 2011 Dec 19;208(13):2705–2716. doi: 10.1084/jem.20110547

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© 2011 Sotolongo et al.

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Figure 8. — Activation of TRIF by poly I:C induces intestinal protective immunity in WT mice by enhancing IFN-β expression in the MLN and macrophage bactericidal activity. (A) Immunoblot analysis of IRF-3 activation (phosphorylation) in the MLNs. Poly I:C–treated (24 and 48 h after injection) and nontreated WT mice are shown. Representative pictures are from three repeated experiments (n = 3). (B) Y. enterocolitica titers in the PPs and MLNs taken 7 d after infection. Pooled data are from three independent experiments (*, P < 0.001 for PPs; *, P < 0.0001 for MLN). Horizontal bars indicate mean. (C) Real-time PCR analysis of IFN-β expression in the MLNs taken 48 h after infection. Combined data from three independent experiments (n = 6; *, P < 0.05). Error bars, SEM. (D) Kaplan Meier survival curve for Trif ^Lps2 mice that received poly I:C (50 µg/mouse s.c) the day before (d-1) Y. enterocolitica infection. Combined data from two independent experiments (n = 6; NS, not significant). (E) Spleen titer results 7 d after orogastrical inoculation with 10⁸ CFU S. typhimurium. Pooled data from two independent experiments (n = 6 each; *, P < 0.001). Error bars, SEM. Horizontal bars indicate mean. (F) Kaplan Meier survival curve for poly I:C–treated mice (n = 10 for control, n = 9 for d-1, n = 7 each for d0 and d+1; *, P < 0.05, combined three independent experiments). (G) Kaplan Meier survival curve for poly I:C–treated (d-1) mice with or without anti-IFNAR antibody. Combined three independent experiments (n = 7 for control, n = 9 each for anti-IFNAR–treated and nontreated groups, n = 5 no-infection; *, P < 0.05). (H) Bactericidal assay of peritoneal macrophages (MOI: 1) with or without 5 µg/ml poly I:C. Combined data from three independent experiments (n = 6 each; *, P < 0.001). Error bars, SEM.