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Determination of 25(OH)D2 and 25(OH)D3 levels in NIST samples.
| NIST -Level 1 | Certified conc. [ng/mL] |
Measured conc. [ng/mL] |
Accuracy [%] |
RSD [%] |
|---|---|---|---|---|
| 25OH-D2 | ND | ND | ND | ND |
| 25OH-D3 | 23.9 | 26.6 | 111.2 | 2.0 |
| NIST -Level 2 | ||||
| 25OH-D2 | 1.7 | 1.7 | 102.0 | 23.7 |
| 25OH-D3 | 12.3 | 12.3 | 100.2 | 2.7 |
| NIST -Level 3 | ||||
| 25OH-D2 | 26.4 | 24.0 | 90.9 | 1.5 |
| 25OH-D3 | 18.5 | 19.4 | 104.7 | 0.6 |
| NIST -Level 4 | ||||
| 25OH-D2 | 2.4 | 2.4 | 98.5 | 5.4 |
| 25OH-D3 | 70.7a | 65.9a | 93.5 | 5.5 |
Value represents the sum of 25(OH)D3 and 3-epi-25(OH)D3
