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. 2011 Dec 21;101(12):2948–2956. doi: 10.1016/j.bpj.2011.11.007

Figure 2.

Figure 2

Detection of PSM/XCer ordered domains in laterally heterogeneous bilayers. The ability of the interface methylated ceramides to form ordered or gel phase domains in multicomponent bilayers was deduced from the 7SLPC-induced fluorescence quenching of tPA as a function of temperature. Representative curves, defined as the relative fluorescence intensity from bilayers with both 7SLPC and tPA (F) over the intensity from bilayers with only tPA (F0), are shown. Sample identification: (A) POPC/PSM/CHL 60/30/10 (by mol), (B) POPC/PSM/CHL 75/15/10, (C) POPC/PSM/PCer/CHL 60/15/15/10 (applies for all ceramide-containing samples), (D) POPC/PSM/NMeCer/CHL, (E) POPC/PSM/OMeCer/CHL, and (F) POPC/PSM/NMeOMeCer/CHL.