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. 2011 Dec 21;101(12):2948–2956. doi: 10.1016/j.bpj.2011.11.007

Figure 4.

Figure 4

Detection of sterol-enriched domains in mixed bilayers containing interface methylated ceramides. The possible displacement of sterol from PSM-rich domains by the ceramide analogs was determined from the 7SLPC-induced fluorescence quenching of CTL as a function of temperature. Quenching was defined as in Fig. 2. Sample identification: (A) POPC/PSM/CHL 60/30/10 (by mol), (B) POPC/PSM/CHL 75/15/10, (C) POPC/PSM/PCer/CHL 60/15/15/10 (applies for all ceramide-containing samples), (D) POPC/PSM/NMeCer/CHL, (E) POPC/PSM/OMeCer/CHL, and (F) POPC/PSM/NMeOMeCer/CHL.