Skip to main content
. Author manuscript; available in PMC: 2012 Jul 8.
Published in final edited form as: Cell. 2011 Jul 8;146(1):148–163. doi: 10.1016/j.cell.2011.05.040

Figure 2. Cav1-dependent extracellular environment regulates cell shape, protrusion number, Rac1 activity and maturation of integrin-dependent adhesions.

Figure 2

(A) FDMs were generated from Cav1WT and KO MEFs (or KO MEFs rescued with Cav1WT or CavY14F) and seeded with unmodified or reconstituted MEFs as indicated. (B) EFs of Cav1WT and KO MEFs seeded in the indicated FDMs. (C) EFs of Cav1KO MEFs rescued by reexpression (+) of Cav1WT or CavY14F and seeded in FDMs generated by similarly-rescued Cav1KO MEFs. (D) Representative Metamorph masks from experiments as in C, with calculated EFs. (E) Representative Cav1KO MEFS seeded in Cav1WT or Cav1KO FDMs; average protrusions per cell are shown. (F) Rac-GTP levels and total Rac expression in Cav1WT MEFs seeded in Cav1WT or KO FDMs. (G) Length of integrin-dependent adhesions (indicated by 9EG7 staining). (H) FRAP analysis of vinculin-GFP–labeled adhesions in transfected cells seeded in the indicated FDMs. (a) Time-lapse sequences showing corresponding regions before photobleaching (PB), immediately after photobleaching (B) and during recovery. (b) Quantification of vinculin-GFP fluorescence recovery for each condition. (c) Percentage of recovery (boxed areas in a) showing the size of the mobile vinculin-GFP fraction. Data are representative of 3 independent experiments (6≤adhesions≤15).