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. Author manuscript; available in PMC: 2012 Jul 8.
Published in final edited form as: Cell. 2011 Jul 8;146(1):148–163. doi: 10.1016/j.cell.2011.05.040

Figure 3. Cav1 promotes patterning and stiffness of 3D matrices, and favors normal tissue architecture.

Figure 3

(A–D) FDMs were generated and after cell extraction were fixed and labeled. The orientation of all thresholded FN fibers was quantified and plotted against the modal angle (set at 0°). (E) Atomic force microscopy was used to plot point-by-point force versus distance along fibers. The chart shows quantification of Young modulus. (F) Skin sections of WT and Cav1KO mice stained with Masson's trichrome and picrosirius red (PR). Polarized light highlights fibrillar collagen. (G) Stromal organization in WT and Cav1KO mammary gland. (a) Multiphoton excitation microscopy coupled to second harmomic generation imaging (MPE-SHG) of intact fixed glands; SHG and autofluorescence signals are shown. Red and yellow arrows mark curled and straight collagen fibers devoid of SHG signal. (b) Mammary gland sections from WT and Cav1KO mice stained as in F. M=Mammary gland, F=Fat cell. (c) Quantification of SHG signal intensity as in a.