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Pulse-chase labeling of rRNA synthesis in strains depleted of Rrp12p. Cells were transferred to glucose medium for 16 h, labeled with [H3] uracil for 1 min, and then chased with a large excess of unlabeled uracil for the times indicated. (A) RNA separated on a 1.2% agarose/formaldehyde gel. (B) RNA separated on an 8% polyacrylamide/urea gel.
