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. 2004 Jan 15;18(2):210–222. doi: 10.1101/gad.1163204

Figure 3.

Figure 3.

Effects of tethering individual EJC proteins inside the Renilla luciferase ORF. (A) Schematic representation of 5′-6bs/Renilla reporter construct and Firefly control. Six MS2-binding sites were inserted 49-nt downstream of the start codon to be in-frame with the Renilla luciferase gene. (B) Levels of 5′-6bs/Renilla reporter mRNA and luciferase activity normalized to Firefly control and represented relative to MS2 alone. The data shown are the average of three to four independent experiments; error bars represent standard deviation. (C) Nuclear and cytoplasmic distribution of 5′-6bs/Renilla reporter and Firefly control mRNA in the presence of various MS2 fusion proteins. RNA was extracted from fractionated nuclear (n) and cytoplasmic (c) compartments and analyzed by RPA (top panel). The percent of total mRNA in the cytoplasm (lower panel).