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. 2004 Jan 15;18(2):210–222. doi: 10.1101/gad.1163204

Figure 4.

Figure 4.

Upf1, Upf2, and Upf3b also enhance the translational yield of 5′-6bs/Renilla mRNA. (A) Schematic representation of 5′-6bs/Renilla and β-globin-6bs constructs carrying identical MS2-binding sites. RPA probes are indicated with thick lines. (B) RPA of 2 and 8 μg of total mRNA extracted from transfected cells. (C) β-globin-6bs mRNA levels in the presence of MS2-fusion proteins relative to MS2 alone. The data reflect the average of two independent experiments; error bars represent the range. (D) Renilla reporter mRNA levels in the presence of MS2-fusion proteins relative to MS2 alone. The data reflect the average of two independent experiments; error bars represent the range. (E) Relative translational yields of Renilla luciferase. Luciferase activities were normalized to mRNA levels and are represented relative to MS2 alone. The data reflect the average of three independent experiments; errors were propagated using standard algorithms.