Table 2. Comparison between co-localization results for entire nuclei and cropped regions.

	Vol Coloc. (µm3)	% Coloc.	% Green	% Red	PC	M Green	M Red	%occupied
Nuceli n = 50
TSA−	6.03±4.49	0.30±0.22	8.48±2	4.73±1.7	−0.020±0.01	0.03±0.02	0.05±0.02	13.21
TSA+ 50	12.23±10	1.01±0.8	12.06±4.2	7.71±2.5	−0.015±0.02	0.07±0.04	0.11±0.07	19.77
TSA+ 100	23.90±19	0.98±0.8	6.86±2.33	9.75±2.7	0.006±0.05	0.12±0.08	0.09±0.07	16.61
ROIs n = 50
TSA−	0.36±0.4	0.55±0.6	13.87±4.3	13.93±5.6	−0.099±0.03	0.03±0.03	0.04±0.03	27.81
TSA+ 50	1 ±0.6	1.8±1.6	19±8.3	19±5.7	−0.13±0.07	0.08±0.06	0.09±0.06	39
TSA+ 100	1.55±2.7	2.15±2.07	17.50±4.5	21.97±5.6	−0.1087±0.08	0.12±0.1	0.1±0.1	40

Data from experiments described in Figure 2 are shown. Cells were labeled and processed as described and 50 whole nuclei of selected cropped regions (ROIs) from each nucleus were analyzed. Cropped region of the same surface area were selected to contain the most highly co-localized nuclear region. A typical example is highlighted in Figure 2Bi (boxed area) in which a regions of substantial chromatin mixing (yellow) lies at the junction of discrete red and green chromatin domains. The total volumes of nuclei and selected cropped regions were 1953±946 µm³ (n = 150) and 65±7 µm³ (n = 150), respectively. No statistically significant differences in the nuclear volume of TSA treated cells were seen.