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. 2011 Dec 21;6(12):e28780. doi: 10.1371/journal.pone.0028780

Figure 2. JCTH-4 causes selective cytotoxicity in OS cells in a time and dose-dependent manner.

Figure 2

Effect of JCTH-4 on cellular viability of OS cells was determined by the WST-1 based colorimetric assay. (A) Saos-2 and (B) U-2 OS cells were treated with JCTH-4 and the WST-1 reagent was used to quantify cell viability. Absorbance was read at 450 nm and expressed as a percent of the control (Me2SO). Values are expressed as mean ± SD from quadruplicates of 3 independent experiments. *p<0.05, **p<0.01, ***p<0.001 versus solvent control (Me2SO). (C) Effect on cellular viability of HOb and NFF cells treated with JCTH-4 compared to Saos-2 and U-2 OS cells after 72 hours. The WST-1 reagent was used to quantify cellular viability. Absorbance was read at 450 nm and expressed as a percent of the solvent control (Me2SO). Values are expressed as mean ± SD from quadruplicates of 3 independent experiments. *p<0.005 versus Saos-2 cells; #p<0.005 versus U-2 OS cells.