Figure 3. CC potentiates the cytotoxicity of JCTH-4 selectively in OS cells.

Effect of JCTH-4 & CC in combination on cellular viability of OS cells was determined by the WST-1 based colorimetric assay. (A) Saos-2 (96 hours), (B) U-2 OS (72 hours), (C) HOb (72 hours), and (D) NFF (72 hours) cells were treated with JCTH-4 and CC and the WST-1 reagent was used to quantify cellular viability. Absorbance was read at 450 nm and expressed as a percent of the solvent control (Me₂SO). Values are expressed as mean ± SD from quadruplicates of 3 independent experiments. *p<0.05, **p<0.01, versus solvent control (Me₂SO); †p<0.001 versus 0.25 µM JCTH-4; ††p<0.01 versus 0.5 µM JCTH-4; #p<0.001 versus 5 µM CC; @p<0.001 versus 0.25 µM JCTH-4+5 µM CC treatment with Saos-2 cells (Figure 3A); &p<0.01 versus 0.5 µM JCTH-4+5 µM CC treatment with U-2 OS cells (Figure 3B).