Figure 5. JCTH-4 and CC do not yield apoptotic morphology in HOb and NFF cells.

Nuclear and cellular morphology of (A) HOb and (B) NFF cells after 72 hours of treatment. Cells were treated with JCTH-4, CC, and solvent control (Me₂SO). Post treatment, the cells were stained with Hoechst 33342 dye. Corresponding phase micrographs are shown below the Hoechst micrographs. Apoptotic morphology is evident in cells with bright and condensed nuclei accompanied by apoptotic bodies, as well as cell shrinkage and blebbing. Images were taken at 400× magnification on a fluorescent microscope. Scale bar = 15 µm. All images are representative of 3 independent experiments.